• EST

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H_erythrogramma

H_tuberculata

• ESTPiper Result

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H_erythrogramma

H_tuberculata

• glimmer Result

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H_erythrogramma

H_erythrogramma Rev

H_tuberculata

H_tuberculata Rev

• ORF Finder

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H_erythrogramma script

H_erythrogramma result

H_tuberculata script

H_tuberculata result

• EC_gene Finder

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H_erythrogramma

H_tuberculata

• blast

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All EST sequence(contig & singlet)

nr database

H_erythrogramma

H_tuberculata

Sea urchin database(Tax=7668)

H_erythrogramma

H_tuberculata

Sorted Result

Union set against Sea utchin

H_erythrogramma

H_tuberculata

• PFP

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H_erythrogramma

H_erythrogramma GO Root
H_erythrogramma GO Slim

H_tuberculata

H_tuberculata GO Root
H_tuberculata GO Slim

• KAAS

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H_erythrogramma

H_tuberculata

• fasta

---

H_erythrogramma

EST

Assembly result

have hit in NR database

have hit in Sea Urchin database

same reading frame in NR, Sea Urchin, glimmer

Glimmer confirmed and translate to protein

Union set(glimmer + tblastx) Protein

H_tuberculata

EST

Assembly result

have hit in NR database

have hit in Sea Urchin database

same reading frame in NR, Sea Urchin, glimmer

Glimmer confirmed and translate to protein

Union set(glimmer + tblastx) Protein

• Clusters information

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Cluster Information

• Species GO comprision result

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Cellular component

Biological process

Molecular function

• Tools

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blast result extract tool